LOCUS       40924_49112        5372 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD020, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 5372)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5372)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    46..51
                     /label=ZraI
                     /note="ZraI"
     misc_feature    49..69
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    70..75
                     /label=XbaI
                     /note="XbaI"
     misc_recomb     76..855
                     /note="S7942NS1-RA; right arm of the homologous
                     recombination site NS1 into the chromosome of Synechococcus
                     elongatus PCC7942 derived from INSD accession NC_007604, 
                     U30252"
     rep_origin      892..1480
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1666..1806)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        1930..1958
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             2006..3193
                     /label=TcR
                     /note="tetracycline efflux protein"
     misc_feature    3197..3286
                     /note="tet-3UTR; 3' UTR of the tetracyclin resistance ORF;
                     from pACYC184 INSD accession X06403"
     CDS             complement(3428..3874)
                     /gene="ndk"
                     /label=ndk
                     /note="Nucleoside diphosphate kinase from Synechococcus 
                     elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805). 
                     Accession#: P50590"
     misc_feature    3880..3885
                     /label=XbaI
                     /note="XbaI"
     misc_feature    4067..4072
                     /label=NheI
                     /note="NheI"
     misc_feature    4127..4132
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    4162..4167
                     /label=NheI
                     /note="NheI"
     misc_feature    4168..4188
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    4186..4191
                     /label=ZraI
                     /note="ZraI"
     CDS             complement(4315..5172)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5173..5277)
                     /label=AmpR promoter
     misc_feature    5303..5308
                     /label=ZraI
                     /note="ZraI"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagcgacgt cgggggcccc
       61 cggggggatt ctagattctg gagcaggaag atgtcgcggg cattagcacc agcggtctgc
      121 caagcctccg ccagccgttg ggtcccttcc gcttgagctt ttccatcttc gacgatacgg
      181 gcggcggccc cccgcgcttc cgcgatcgcc cgtttacaag ctgcctcagc tggggcgatc
      241 acatcggctt gaagttgctg ctgcacctgt ttgatccgct cctgctgcac agggagttct
      301 gcttggctac gagcgacttc ggtagcaatg tccgcttcag cttcggccac caccgcttcg
      361 cgccgcgtca acgcatcctg aatccggcgc tcggcctcgg cttgggcgat cgctacatcg
      421 cgatcgatcc gacgcagggc cgtgatcttg tcattttcgg ccgtttggat cgcagaggca
      481 gcctgggcat cggcttcagc aattcgggca tctcgctgca gatcagcccg ctgcttgcgt
      541 ccactagccg agagataacc gacctcatcg gaaatgttct ggacttgcag cgtatcgagg
      601 actagaccca gctgctcaag gtcatcctcc gcctcttcca gcagactttt ggcaaaggca
      661 attttgtcct cgttgatctg ctccggcgtg aggctggcta aaacaccacg caagttgcct
      721 tcgagggtct ccttggcaat ttgctcgatt tccttacggt ttttgccaag cagccgctcg
      781 atcgcgttgt ggatggtcgg ttcttcccca gcaatcttga tattggcaac gccttcaaca
      841 gtcaggggaa tgccgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      901 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      961 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1021 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1081 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1141 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1201 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1261 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1321 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1381 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1441 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1501 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     1561 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     1621 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     1681 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     1741 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     1801 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     1861 gcatccgctt acagacaagc tccgccacct gaagtcagcc ccatacgata taagttgtaa
     1921 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     1981 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     2041 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     2101 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     2161 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     2221 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     2281 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     2341 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     2401 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     2461 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     2521 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     2581 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     2641 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     2701 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     2761 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     2821 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     2881 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     2941 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     3001 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcattggacc
     3061 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     3121 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     3181 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     3241 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccagcgg cggagcgctg
     3301 ctttcttggc aagcggtcgc cagccccaac gccagggctg ccagcccgaa acagcggggc
     3361 aaggcagctt ggaagggcga tcgcagcacg ggcatggcaa tgtctctctg aaggaatgca
     3421 gaccttattc gtacagccag ggttgaatcg tgggggtcca atcacttagc tctgctgggc
     3481 taaaccagag agcaatttcc tgttgtgctg tttcgattgc atccgagcca tggatgatgt
     3541 tgcggccaat attgacacca aaatcaccac ggatggtgcc cggttctgcc gtcagcggat
     3601 tggtagcgcc gatcaacttg cgagcagccg ccacaacgcc ttcgccttcc aagacgatcg
     3661 ccacgatcgg cccagaggtg atgaactcga cgaggccatt gaagaagggg cgctcgcggt
     3721 ggacagcata gtgctgttcg gccagctcgc gactgggctt cagctgcttt aggcccacca
     3781 gtttgaagcc tttttgctca aagcggccga tgatcgtacc gaccaaaccc cgctgaacgc
     3841 catcgggctt gatggcaata aatgtgcgtt ccacagacat ctagatagtc ctcaagacga
     3901 ggcaagcatt gagcttgcct tcctatggtt cgggatcact gggattcttg acaagcgatc
     3961 gcggtcacat cgctatctct taggacttcg cagcgggcga gtcggattga cccggtaggg
     4021 atttcgccag atcaatgccc gtggtttgtt tcagcttctc cagcaagcta gcgatttggg
     4081 tagcgctgcc ttccccttcg ccaatcacag tgatcgactc cacgtcgata tctggcacgg
     4141 tgcctgaaag cgtgacgagc agctagcatc gcgcgcgcgc gcgcggacgt cctcacgtta
     4201 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4261 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4321 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4381 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4441 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     4501 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4561 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4621 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4681 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4741 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4801 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4861 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4921 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4981 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     5041 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     5101 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5161 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5221 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5281 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     5341 taaaaatagg cgtatcacga ggccctttcg tc
//
